Week 9: My Phages program investigation

“Bacteriophages are virus that have DNA or RNA as genetic material, they infect Bactrian cells specific and they reproduce by lysis” (Makarov Roman 2008). This investigation was about found new bacteriophages in different soil samples on Puerto Rico and then send it to HHMI to be sequenced. First we have to take the soil samples and take them to the laboratory. Before this we proceed to worked with the soil samples to know if it had or no bacteriophages and if it infects  Bacillus or Msmeg. This process was done by the techniques of characterization and isolation.

The purpose of this investigation was too sequence the DNA of a new bacteriophage.  As result of this investigation, I found the presence of a bacteriophage and I called it Ocsicnarf. Ocsicnarf was a phage that I have to purify several time because it was mixed with other types of phages. This phage was found in Cayey P.R. Doing this investigation I got so much skills like get the knowledge and practice of what is the aseptic technique,  how to isolate an specific thing (in this case a phage), purification, centrifugation, electrophoresis, fluting a petri dish and equal, manage the laboratory equipment like the pipet, lighter, centrifuge, micropipette.

                                                                 Image of Ocsicnarf